The "hang-drop" method allows the examination of a microorganism in its natural environment. This method allows the organism to maintain its natural shape and size. It also allows one to determine if the organism is motile (capable of moving itself).
How do you distinguish between Brownian movement and true mobility?
By using the "hang-drop" method, one will be able to determine if the organism being studied is motile (capable of moving itself).
Equipment:
Using a wooden toothpick, apply vaseline around the edge of the depression on a depression slide.
Using an inoculating loop, transfer one or two loopfuls of bacterial broth or hay infusion onto a cover slip.
Invert the depression slide over the suspension on the coverslip. Press on the slide gently to form a seal.
Lift the preparation and turn it right side up and examine it with a microscope under low power, then switch to high power.
Review with the class proper laboratory procedures for handling bacteria and using lab equipment. Circulate during the experiment to supervise and offer assistance where needed.
Remember to use proper lab procedures. Clean your work station when finished. Thoroughly wash your hands.
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